Multimodal intravascular imaging for diagnosis and monitoring of atherosclerosis

Atherosclerosis begins in childhood and progresses throughout life culminating in coronary artery disease (CAD), cerebrovascular disease (CVD), and peripheral artery disease (PAD) in middle-aged and older individuals. The combined mortality rate in 2006 for all cardiovascular disease was 263 per 100,000, which accounted for 1 in 3 deaths in the US. The majority could be traced to the affects of atherosclerosis. At present there is no cure for atherosclerosis.

Intravascular Optical Coherence Tomography (OCT) is currently being used clinically during catheterization procedures for aiding in the deployment of stents. Beyond the current clinical application there is a desire to develop some imaging platform for identifying atherosclerotic plaques that may have a propensity to rupture causing a sudden coronary event (heart attack) or simply to assess risk for such events. Such plaques are thought to have very distinct morphological and biochemical features. Unfortunately OCT is not capable of directly measuring the biochemical features. In order to overcome this we have been developing a multimodal technique which pairs fluorescence lifetime imaging with OCT.  We recently published a pilot study indicating that we could readily identify the collagen and lipid content of plaques; high lipid content is associated with plaques that would be considered vulnerable. We are currently developing a multimodal intravascular catheter that would allow us to do a similar study in an in vivo animal model and eventually in live humans undergoing cardiac catheterization.  This work is being done in collaboration with our collegues  at the Texas Heart Institute.

 


RElevant Publications

  1. J. A. Jo, J. Park, P. Pande, S. Shrestha, M. J. Serafino, J. Rico Jimenez, F. Clubb, B. Walton, M. Buja, J. E. Phipps, M. D. Feldman, J. Adame, and B. E. Applegate, “Simultaneous Morphologic and Biochemical Endogenous Optical Imaging of Atherosclerosis,” Eur Heart J Cardiovasc Imaging, (2015) in press PMID: 25722204    
  2. J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, "Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging microscopy," Atherosclerosis  220, 394-401 (2012) PMID: 22138141    
  3. J. Park*, J. A. Jo, S. Shrestha, P. Pande, Q. Wan, and B. E. Applegate, "A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization," Biomed Opt Express 1, 186-200 (2010) PMID: 21258457    
  4. S. Shrestha, B. E. Applegate, J. Park, X. Xiao*, P. Pande, and J. A. Jo, "High-Speed Multispectral Fluorescence Lifetime Imaging Implementation for in vivo applications," Opt Lett 35, 2558-2560 (2010) PMID: 20680057